Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PU.1 is a mediator of the reactive stromal response associated with castration-resistant prostate cancer.

Communications biology·2026
Same author

'BJUI Clinical Dilemma': Recurrent high-grade non-muscle-invasive bladder cancer in 2026.

BJU international·2026
Same author

Oncological Outcomes of Epirubicin-based Hyperthermic Intravesical Chemotherapy in Bacillus Calmette-Guérin (BCG)-naïve Patients and Patients with BCG Failure: A Multicenter Retrospective Observational Study.

European urology open science·2026
Same author

Incidental Prostate Cancer Is an Uncommon but Clinically Non-negligible Cause of Death in Selected Long-term Survivors of Urothelial Carcinoma-Oncological Outcomes and a Proposed Follow-up Strategy from a Tertiary Referral Center.

European urology open science·2026
Same author

Dose-intensified Versus Conventional-dose Salvage Radiotherapy for Biochemically Recurrent Prostate Cancer After Prostatectomy: Long-term Data from the SAKK 09/10 Randomised Phase 3 Trial.

European urology·2026
Same author

An Unusual Inguinal Mass in a 76-Year-old Patient With Suspected Cryptorchidism.

Urology·2025

Related Experiment Video

Updated: Apr 22, 2026

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
09:43

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts

Published on: August 1, 2025

977

Ceramic foam plates: a new tool for processing fresh radical prostatectomy specimens.

Tatjana Vlajnic1, Martin Oeggerli, Cyrill Rentsch

  • 1Institute of Pathology, University Hospital Basel, Schoenbeinstrasse 40, 4031, Basel, Switzerland, tatjana.vlajnic@usb.ch.

Virchows Archiv : an International Journal of Pathology
|October 18, 2014
PubMed
Summary

A new method using ceramic foam plates effectively processes fresh prostate cancer tissue from radical prostatectomy specimens. This technique preserves tissue integrity for biobanking and xenograft models, aiding advanced prostate cancer research.

More Related Videos

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
07:16

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

13.8K
Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking
07:34

Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking

Published on: November 17, 2023

1.5K

Related Experiment Videos

Last Updated: Apr 22, 2026

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
09:43

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts

Published on: August 1, 2025

977
Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
07:16

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

13.8K
Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking
07:34

Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking

Published on: November 17, 2023

1.5K

Area of Science:

  • Urology
  • Oncology
  • Biotechnology

Background:

  • Procuring fresh prostate cancer tissue is vital for biobanking and xenograft models in advanced prostate cancer research.
  • Handling fresh radical prostatectomy specimens is challenging due to tissue properties and difficulty in identifying cancer foci.
  • Current methods may compromise tissue integrity for subsequent diagnostic and research applications.

Purpose of the Study:

  • To develop and evaluate a novel method for processing fresh whole mount sections of radical prostatectomy specimens.
  • To ensure precise identification and collection of fresh tumor tissue without compromising diagnostic assessment.
  • To facilitate biobanking and xenograft model generation for preclinical studies in advanced prostate cancer.

Main Methods:

  • Developed a technique using ceramic foam plates to process fresh whole mount sections from radical prostatectomy specimens.
  • Processed 49 radical prostatectomy specimens, sectioning them from apex to base.
  • Verified putative carcinoma foci via frozen section analysis before processing.
  • Fixed fresh whole mount slices between two ceramic foam plates overnight.
  • Evaluated tissue preservation using hematoxylin and eosin (H&E) staining, immunohistochemistry, fluorescence, and silver in situ hybridization (FISH and SISH).

Main Results:

  • No morphological artifacts were observed on H&E stained whole mount sections processed with ceramic foam plates.
  • Histological architecture was fully retained in the processed tissue slices.
  • Immunohistochemistry, FISH, and SISH analyses yielded excellent quality results.
  • The method allowed precise identification and collection of fresh tumor tissue.

Conclusions:

  • Fixing whole mount tissue slices between ceramic foam plates is an excellent method for processing fresh radical prostatectomy specimens.
  • This approach enables precise identification and collection of fresh tumor tissue.
  • The method does not compromise further diagnostic analysis and supports biobanking and xenograft model generation.