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Updated: Apr 22, 2026
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
Published on: June 16, 2018
Autophagy activity in pulmonary metastatic tumor tissues from colorectal cancer: a pilot study
Young Sam Kim1, Ju Hye Shin1, Mi Kyung Bae2
1Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Purpose:
Autophagy has been reported to be involved in treatment failure in tumor. We aimed to evaluate autophagy activity in tumor tissue and compare them between the recurrence and non-recurrence groups.
Materials And Methods:
We analyzed expressions of autophagy-related proteins in tumor tissues which were obtained from pulmonary metastases of colorectal cancer patients by Western blot. We also analyzed autophagosomes by transmission electron microscopy.
Results:
Tumor tissues from recurrence group showed increased levels of LC3B-II, decreased levels of p62/SQSTM1, and also a marked accumulation of autophagosomes compared with tissues from non-recurrence group.
Conclusion:
The present study suggests that autophagy may be associated with treatment failure of metastatic colorectal cancer.
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