Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same journal

Rgg/SHP transcriptional regulators, RaS-RiPPs, and their impacts in streptococci.

F1000Research·2026

Same journal

Functional impact and quality of life after total hip arthroplasty for hip osteoarthritis. A retrospective study of 200 cases.

F1000Research·2026

Same journal

Exploring potential strategies to enhance memory and cognition in aging mice.

F1000Research·2026

Same journal

Construction an Implicit Block Multi-Steps Approach for Solving Sixth-Order Fractional Differential Equations.

F1000Research·2026

Same journal

Kansei Engineering in the Evolving Service Sector: A Decade of Insights.

F1000Research·2026

Same journal

A Safety-First Mindset: Role of Patient Safety Culture in Enhancing Healthcare Workers' Emotional Intelligence.

F1000Research·2026

Search research articles

Related Experiment Video

Updated: Apr 22, 2026

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Commercial antibodies and their validation.

¹Everest Biotech Ltd, Upper Heyford, OX25 5HD, UK.

|October 29, 2014

Summary

Scientists face challenges with commercial research antibodies due to inactivation, flawed materials, and inconsistent validation. Choosing validated antibodies for specific applications is crucial for experimental success and reducing wasted resources.

Area of Science:

Immunology
Biotechnology
Life Sciences Research

Background:

Commercial research antibodies are widely used but suffer from a general lack of trust among scientists.
Numerous issues contribute to antibody failure, including inactivation, flawed biological materials, assay problems, and inconsistent product representation across catalogs.
Wasted resources are common due to repeated purchase and testing of unreliable antibodies.

Purpose of the Study:

To highlight the common pitfalls and challenges associated with the use of commercial research antibodies.
To emphasize the importance of proper validation and application-specific antibody selection.
To address the lack of trust in commercial antibody products within the scientific community.

Main Methods:

The study is a review and analysis of common issues affecting research antibody performance.

More Related Videos

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Published on: May 18, 2021

Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Published on: January 26, 2019

Related Experiment Videos

Last Updated: Apr 22, 2026

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Published on: May 18, 2021

Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Published on: January 26, 2019

It identifies factors contributing to antibody failure in experimental settings.

It discusses the implications of batch-to-batch variability and outdated quality control data.

Main Results:

Commercial antibodies often fail due to inactivation, material flaws, or incorrect experimental conditions.
Inconsistent product specifications and batch variations lead to unreliable results.
Antibodies validated for specific applications offer a higher chance of experimental success.

Conclusions:

A significant lack of trust exists in commercial research antibodies, stemming from multiple failure points.
Scientists must be aware of potential pitfalls such as antibody inactivation, flawed reagents, and batch variability.
Selecting antibodies with proven performance in the intended application and platform is essential to mitigate experimental failures and resource waste.