Related Experiment Video
Updated: Apr 21, 2026

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones
Published on: February 5, 2018
Nootkatone
Robin-Hagen Leonhardt1, Ralf G Berger
1Institut für Lebensmittelchemi, Gottfried Wilhelm Leibniz Universität Hannover, Callinstraße 5, 30167, Hannover, Germany, rg.berger@lci.uni-hannover.de.
Abstract:
The continuing interest in the sesquiterpene ketone (+)-nootkatone is stimulated by its strong grapefruit-like odor and numerous further bioactivities. Also numerous were the attempts to chemosynthesize or biotechnologically produce the compound. Cytochrome P450 enzymes from bacteria and fungi were intensively studied and expressed in Escherichia coli and in more food compatible hosts, such as Saccharomyces cerevisiae. The lipoxygenase-catalyzed generation was demonstrated using an enzyme from several Pleurotus species. Laccases required artificial mediators for an efficient catalysis. More recently, plant valencene synthases were expressed in microbial hosts. Combined with an endogenous farnesyl diphosphate delivery pathway and a valencene oxidase, this approach opened access to high yields of nootkatone possessing the appreciated attribute of "natural" according to present food legislation. Little biochemical engineering was carried out on the novel recombinant strains, leaving many options for future improved bioprocesses.
More Related Videos
07:30A Direct, Regioselective and Atom-Economical Synthesis of 3-Aroyl-N-hydroxy-5-nitroindoles by Cycloaddition of 4-Nitronitrosobenzene with Alkynones
Published on: January 21, 2020
09:26Functional Magnetic Resonance Spectroscopy at 7 T in the Rat Barrel Cortex During Whisker Activation
Published on: February 8, 2019
Related Concept Videos
Nociception
IUPAC Nomenclature of Ketones
Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists
2° Amines to N-Nitrosamines: Reaction with NaNO2
Nitriles to Ketones: Grignard Reaction
The mechanism begins with a nucleophilic attack by the...
Nitrosation of Enols