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Bialaphos resistance as a dominant selectable marker in Neurospora crassa
J Avalos1, R F Geever, M E Case
1Department of Genetics, University of Georgia, Athens 30602.
Current Genetics
|December 1, 1989
Abstract:
Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/l in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.