Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microenvironments01:22

Microenvironments

43
Microorganisms inhabit highly localized spaces known as microenvironments, which are defined by distinct physical and chemical characteristics. These include oxygen concentration, pH, temperature, light availability, and nutrient levels. The conditions within a microenvironment can differ markedly from those in the surrounding area and significantly influence microbial growth, metabolism, and community structure.Microenvironments often display sharp physicochemical gradients over small spatial...
43

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GDF15 (Growth/Differentiation Factor-15) Expression in Human Adipose Tissue and in Adipocyte Cell Lines.

Biomedicines·2026
Same author

Obesity- and Glucose-Dependent Differential Autophagy Marker Expression in Adipose Tissues and Adipocytes.

Obesity science & practice·2026
Same author

Oral losartan yields subtherapeutic airway exposure compared to nebulized delivery and fails to improve mucociliary clearance in cystic fibrosis.

Frontiers in pharmacology·2026
Same author

In-situ product recovery in microfluidic bioreactors.

Current opinion in biotechnology·2026
Same author

Establishment of a Single-Cell Minimal Medium: A Case Study for Microfluidic Cultivation of Corynebacterium glutamicum.

Biotechnology and bioengineering·2026
Same author

Age-Dependent Systemic Regulation of C1q/TNF-Related Protein 3 and Progranulin in Patients with Cystic Fibrosis: Biomarkers or Therapeutic Targets?

Biomedicines·2026

Related Experiment Video

Updated: Apr 21, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

11.6K

Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes.

Christian Dusny1,2, Andreas Schmid1,2

  • 1Department of Solar Materials, Helmholtz Centre for Environmental Research GmbH - UFZ, Permoserstr. 15, Leipzig, DE 04318, Germany.

Environmental Microbiology
|October 21, 2014
PubMed
Summary

Microfluidic single-cell analysis enables precise environmental control, overcoming limitations of traditional cell cultures. This technology allows detailed study of individual cell physiology and growth rates, revealing insights into biological individuality.

More Related Videos

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

3.3K
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

14.7K

Related Experiment Videos

Last Updated: Apr 21, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

11.6K
Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

3.3K
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

14.7K

Area of Science:

  • Cell Biology
  • Biophysics
  • Microfluidics

Background:

  • Cells are fundamental biological units exhibiting individuality in traits and responses.
  • Traditional population-based cultures obscure single-cell dynamics and environmental influences.
  • Lack of environmental control and population averaging limit understanding of phenotypic individuality.

Purpose of the Study:

  • To explore the interplay between cellular physiology and environment at the single-cell level.
  • To review biological and physical fundamentals governing cell function and environment.
  • To highlight the significance of single-cell growth rates in understanding cellular physiology.

Main Methods:

  • Utilizing microfluidic single-cell analysis for precise environmental manipulation.
  • Cultivating and analyzing single cells in controlled extracellular environments.
  • Time-resolved analysis of single-cell dynamics.

Main Results:

  • Microfluidic platforms enable decoupling of stochastic processes and extracellular contributions.
  • Precise environmental control allows for detailed observation of individual cell behavior.
  • Single-cell growth rates serve as key indicators of global cellular physiology.

Conclusions:

  • Microfluidic single-cell cultivation offers unique opportunities to study fundamental biological questions.
  • Growth rate analysis in controlled microenvironments provides deep insights into cellular individuality.
  • This approach advances understanding in cell biology and biotechnology.