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Related Experiment Videos

Ca2+-ATPase activity in the human lens.

D Borchman1, C A Paterson, N A Delamere

  • 1Department of Ophthalmology and Visual Sciences, University of Louisville School of Medicine, Kentucky.

Current Eye Research
|October 1, 1989
PubMed
Summary
This summary is machine-generated.

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Human lens membranes show calcium-dependent ATPase activity, primarily in the epithelium and cortex, with activity decreasing with age. This suggests age-related modifications in lens membrane function.

Area of Science:

  • Ophthalmology
  • Biochemistry
  • Cell Biology

Background:

  • The human lens maintains transparency through precise ion and water balance.
  • Membrane-bound ATPases play a crucial role in cellular energy metabolism and ion transport.
  • Understanding lens ATPase activity is vital for comprehending age-related changes and potential pathologies like cataracts.

Purpose of the Study:

  • To investigate the characteristics of calcium-dependent ATPase (Ca2+-ATPase) activity in human lens membranes.
  • To determine the distribution and calcium sensitivity of Ca2+-ATPase in different regions of the human lens.
  • To explore potential age-related alterations in lens Ca2+-ATPase activity and membrane composition.

Main Methods:

  • Preparation of membrane-rich fractions from paired human lenses, preserving ATPase activity.

Related Experiment Videos

  • Modification of lipid:protein ratio by increasing phospholipid content.
  • Assay of ATPase activity across a range of calcium concentrations in epithelial, cortical, and nuclear membrane preparations.
  • Comparison of Ca2+-ATPase activity and calcium sensitivity between different lens regions and age groups.

    • Main Results:

    • Ca2+-ATPase activity was detected in human lens epithelial and cortical membrane preparations.
    • Calcium half-maximal stimulation concentration was approximately 10(-6) M for both epithelium and cortex.
    • No detectable Ca2+-ATPase activity was found in the lens nuclear region.
    • Increasing age correlated with a shift in Ca2+-ATPase sensitivity and an overall increase in activity in lens epithelial cells.

    Conclusions:

    • Human lens epithelium and cortex possess a calcium-sensitive Ca2+-ATPase.
    • Lens nuclear region lacks significant Ca2+-ATPase activity.
    • Age-related changes in human lens membranes likely involve modifications to Ca2+-ATPase activity and sensitivity.