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Identification of microRNAs in human plasma

Bram C van der Eerden1, Rodrigo D Alves, Christel E Kockx

  • 1Department of Internal Medicine, Erasmus MC, Room Ee585b, 3000 DR, 1738, Rotterdam, The Netherlands, b.vandereerden@erasmusmc.nl.

Methods in Molecular Biology (Clifton, N.J.)
|October 22, 2014
PubMed

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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