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Updated: Apr 21, 2026
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
Published on: November 2, 2018
Fluorescence recovery after photobleaching (FRAP): acquisition, analysis, and applications
Michael Carnell1, Alex Macmillan, Renee Whan
1Biomedical Imaging Facility, Lowy Cancer Research Centre, University of New South Wales, High Street, Kensington, Sydney, NSW, 2052, Australia.
Abstract:
A significant number of biological processes occur at, or involve cellular membranes, including; cell adhesion, migration, endocytosis, signal transduction, and many biochemical reactions involving membrane anchored scaffolds. Each process involves a complex arrangement of interacting molecules whose location in space and time influence the outcome of the event. In this protocol we discuss the application of fluorescence recovery after photobleaching (FRAP) to study the dynamics of membrane associated molecules. We discuss the principles, acquisition and the analysis of FRAP data and address issues surrounding its interpretation.
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01:19Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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