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A yeast pheromone-based inter-species communication system.

Stefan Hennig1, André Clemens, Gerhard Rödel

  • 1Institut für Genetik, Technische Universität Dresden, 01062, Dresden, Germany, Stefan.Hennig1@tu-dresden.de.

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Scientists engineered yeast species Saccharomyces cerevisiae and Schizosaccharomyces pombe for inter-species communication. This system uses pheromones to enable controlled cell-cell signaling between these two distinct yeast types.

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Area of Science:

  • Synthetic Biology
  • Microbiology
  • Cell Biology

Background:

  • Yeast species Saccharomyces cerevisiae and Schizosaccharomyces pombe possess distinct mating response pathways.
  • Pheromones, such as α-factor and P-factor, are key signaling molecules in yeast mating.
  • Controlled inter-species communication in yeast has potential applications in synthetic biology.

Purpose of the Study:

  • To establish a proof-of-principle for inter-species cell-cell communication between Saccharomyces cerevisiae and Schizosaccharomyces pombe.
  • To engineer yeast species to recognize and respond to heterologous pheromones.
  • To demonstrate controlled signaling across species using modified pheromone systems.

Main Methods:

  • Engineering of authentic and chimeric pheromone-encoding genes for heterologous expression.
  • Transformation of Saccharomyces cerevisiae to express P-factor and Schizosaccharomyces pombe to express α-factor.
  • Analysis of cell cycle arrest (G1), morphological changes (shmoo effect), and gene expression (FIG1, rep1, sxa2 promoters) in response to pheromones.

Main Results:

  • Schizosaccharomyces pombe expressing α-factor induced G1 arrest, shmoo effect, and FIG1 promoter activation in Saccharomyces cerevisiae.
  • Saccharomyces cerevisiae expressing P-factor induced G1 arrest, mating morphology, and rep1/sxa2 promoter activation in Schizosaccharomyces pombe.
  • Heterologous pheromones were correctly processed and secreted in active forms by the recipient species.

Conclusions:

  • The species-specific pheromone systems of yeast can be successfully exploited for controlled inter-species communication.
  • Engineered yeast strains can act as recipients and senders in a heterologous signaling system.
  • This work provides a foundation for developing more complex synthetic microbial consortia.