Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.7K
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

1.3K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.3K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-time, depth-resolved, in vivo multiphoton fluorescence lifetime imaging microscopy of agricultural herbicide treatments in plants.

Optics express·2024
Same author

Spatial and Depth Profiling of Agricultural Formulations in Leaf Tissue Using LAESI Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2024
Same author

CHRISTMAS: Chiral Pair Isobaric Labeling Strategy for Multiplexed Absolute Quantitation of Enantiomeric Amino Acids.

Analytical chemistry·2023
Same author

6-Plex <i>md</i>SUGAR Isobaric-Labeling Guide Fingerprint Embedding for Glycomics Analysis.

Analytical chemistry·2023
Same author

Focus on Next Generation Mass Spectrometry Omics Technologies, in Honor of Dr. Erin Baker, Recipient of the 2022 ASMS Biemann Medal.

Journal of the American Society for Mass Spectrometry·2023
Same author

Dermal effects and pharmacokinetic evaluation of the lidocaine/prilocaine cream in healthy Chinese volunteers.

BMC pharmacology & toxicology·2023

Related Experiment Video

Updated: Apr 21, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

13.0K

Optimization and comparison of multiple MALDI matrix application methods for small molecule mass spectrometric

Erin Gemperline1, Stephanie Rawson, Lingjun Li

  • 1Department of Chemistry, University of Wisconsin-Madison , 1101 University Avenue, Madison, Wisconsin 53706, United States.

Analytical Chemistry
|October 22, 2014
PubMed
Summary

The automatic sprayer method for matrix application in MALDI mass spectrometric imaging (MSI) detects more metabolites and offers better reproducibility for Medicago truncatula root nodules compared to airbrush and sublimation. This technique enhances metabolite profiling in plant tissues.

More Related Videos

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
09:59

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

18.4K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

8.9K

Related Experiment Videos

Last Updated: Apr 21, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

13.0K
MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
09:59

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

Published on: March 5, 2014

18.4K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

8.9K

Area of Science:

  • Analytical Chemistry
  • Plant Science
  • Biotechnology

Background:

  • Matrix application is crucial for matrix-assisted laser desorption/ionization (MALDI) experiments.
  • MALDI mass spectrometric imaging (MSI) is a powerful tool for analyzing metabolite distribution in biological samples.
  • Understanding metabolite profiles in plant root nodules is important for legume biology and agriculture.

Purpose of the Study:

  • To systematically evaluate and compare three matrix application techniques for MALDI MSI of endogenous metabolites in Medicago truncatula root nodules.
  • To optimize airbrush, automatic sprayer, and sublimation methods for metabolite detection using 2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (CHCA) matrices.
  • To assess analytical reproducibility and analyte diffusion for each matrix application method.

Main Methods:

  • Optimization of airbrush, automatic sprayer, and sublimation for MALDI MSI in positive ionization mode.
  • Use of DHB and CHCA as MALDI matrices for metabolite analysis.
  • Comparative analysis of metabolite detection, analytical reproducibility, and analyte diffusion across methods.
  • Investigation of humidity effects on sublimation for metabolite detection.

Main Results:

  • The automatic sprayer method, particularly with DHB, detected approximately double the number of metabolites compared to sublimation and airbrush.
  • The automatic sprayer demonstrated superior reproducibility and reduced analyte diffusion versus the airbrush method.
  • Sublimation provided high spatial resolution and reproducibility but detected fewer high m/z metabolites (500-1000 m/z).
  • Humidified sublimation enhanced detection of higher mass metabolites but increased diffusion of lower mass analytes.
  • With CHCA, both the optimized automatic sprayer and humidified sublimation doubled metabolite detection compared to the standard airbrush method.

Conclusions:

  • The automatic sprayer technique is highly effective for MALDI MSI of plant metabolites, offering enhanced detection and reproducibility.
  • Sublimation provides excellent spatial resolution but may require optimization (e.g., humidity) to improve detection of a wider range of metabolites.
  • The choice of matrix application technique significantly impacts the outcome of MALDI MSI experiments for plant metabolite profiling.