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Phospholipase A2-induced pleural inflammation in rats
B M Weichman1, J W Berkenkopf, L A Marshall
1Division of Immunopharmacology, Wyeth-Ayerst Research, Princeton, NJ 08543.
International Journal of Tissue Reactions
|January 1, 1989
Summary
Phospholipase A2 (PLA2) from Naja mocambique mocambique snake venom causes inflammation in rat pleural cavities. This enzyme is also released during experimental pleurisy, though its exact role in inflammation requires further study.
Area of Science:
- Biochemistry
- Toxicology
- Immunology
Background:
- Phospholipase A2 (PLA2) is an enzyme involved in inflammatory processes.
- The specific role of PLA2 in pleural inflammation is not fully understood.
Purpose of the Study:
- To investigate the effects of exogenous Naja mocambique mocambique phospholipase A2 (PLA2) on rat pleural inflammation.
- To determine if endogenous PLA2 is released during experimental pleurisy.
Main Methods:
- Rats were injected with varying doses of Naja mocambique mocambique PLA2 into the pleural cavity.
- Cellular infiltration and fluid accumulation were measured over time.
- PLA2 activity in pleural exudate was quantified during reverse passive Arthus reaction (RPAR) induced pleurisy.
Main Results:
- Exogenous PLA2 induced dose- and time-dependent pleural fluid accumulation and cellular infiltration.
- Neutrophilic infiltration occurred at 2-6 hours, followed by mononuclear infiltration at 6-24 hours.
- Endogenous PLA2 activity was detected in pleural exudate during RPAR-induced pleurisy, but its elevation lagged behind eicosanoid biosynthesis and plasma extravasation.
Conclusions:
- Exogenous PLA2 can induce pleural inflammation in rats.
- PLA2 is released endogenously during experimental pleurisy.
- The precise role of PLA2 in the inflammatory response warrants further investigation.