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Related Experiment Video

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Cell-cell proximity effects in multi-cell electroporation.

Brian E Henslee1, Andrew Morss2, Xin Hu3

  • 1Department of Chemical and Biomolecular Engineering, The Ohio State University , Columbus, Ohio 43210, USA.

Biomicrofluidics
|October 22, 2014
PubMed
Summary

The presence of a second cell alters a cell's electroporation threshold field by 5%-10%. Cell orientation relative to the electric field significantly impacts this effect, with parallel alignment shielding and perpendicular alignment enhancing field effects.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Electrochemistry

Background:

  • Electroporation studies of cell suspensions are complex.
  • Previous models treated cells as independent entities.
  • Quantitative modeling of dense cell suspensions is challenging.

Purpose of the Study:

  • To investigate how a nearby cell influences the electroporation threshold field of another cell.
  • To quantify the effect of cell-cell proximity on electropermeabilization.
  • To develop a model predicting these effects.

Main Methods:

  • Utilized optical tweezers for precise two-cell positioning in a fluidic electroporation device.
  • Measured the threshold electric field for electropermeabilization.
  • Employed finite element simulations (3-layer model, contact resistance methods) for electrostatic potential analysis.
  • Recorded video of the electropermeabilization process.

Main Results:

  • A nearby cell (nearly touching) alters the threshold field by approximately 5%-10%.
  • Cells aligned parallel to the electric field exhibit shielding effects.
  • Cells oriented perpendicular to the field experience enhanced electric field effects.
  • Model predictions showed good agreement with experimental measurements.

Conclusions:

  • Cell-cell interactions significantly modify electropermeabilization thresholds.
  • The orientation of cells relative to the electric field is a critical factor.
  • The dynamics of electropermeabilization are crucial for understanding even simple two-cell systems.