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Updated: Apr 21, 2026

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Published on: December 24, 2016
Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines
Romain Banchereau1, Nicole Baldwin1, Alma-Martina Cepika1
1Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, Texas 75204, USA.
Abstract:
The mechanisms by which microbial vaccines interact with human APCs remain elusive. Herein, we describe the transcriptional programs induced in human DCs by pathogens, innate receptor ligands and vaccines. Exposure of DCs to influenza, Salmonella enterica and Staphylococcus aureus allows us to build a modular framework containing 204 transcript clusters. We use this framework to characterize the responses of human monocytes, monocyte-derived DCs and blood DC subsets to 13 vaccines. Different vaccines induce distinct transcriptional programs based on pathogen type, adjuvant formulation and APC targeted. Fluzone, Pneumovax and Gardasil, respectively, activate monocyte-derived DCs, monocytes and CD1c+ blood DCs, highlighting APC specialization in response to vaccines. Finally, the blood signatures from individuals vaccinated with Fluzone or infected with influenza reveal a signature of adaptive immunity activation following vaccination and symptomatic infections, but not asymptomatic infections. These data, offered with a web interface, may guide the development of improved vaccines.
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