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Related Concept Videos

In-vitro Mutagenesis01:16

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

Updated: Apr 21, 2026

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
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Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

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Making designer mutants in model organisms.

Ying Peng1, Karl J Clark2, Jarryd M Campbell2

  • 1Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA peng.ying@mayo.edu ekker.stephen@mayo.edu.

Development (Cambridge, England)
|October 23, 2014
PubMed
Summary
This summary is machine-generated.

Genome engineering tools like zinc-finger nucleases (ZFNs), TALENs, and CRISPR/Cas9 enable precise DNA modification. These technologies are revolutionizing the study of developmental biology in model organisms and beyond.

Keywords:
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems (Cas9)Genome engineeringModel organismsTranscription activator-like effector nuclease (TALEN)Zinc finger nuclease (ZFN)

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Area of Science:

  • Genomics
  • Developmental Biology
  • Molecular Biology

Background:

  • Recent advancements in genome engineering technologies have significantly enhanced our ability to study complex eukaryotic genomes.
  • Tools such as zinc-finger nucleases (ZFNs), TALENs, and CRISPR/Cas9 have become increasingly sophisticated and accessible.

Purpose of the Study:

  • To review the common approaches and applications of genome engineering tools in developmental biology.
  • To highlight the impact of these tools on established and emerging model systems.

Main Methods:

  • Review of literature on genome engineering techniques.
  • Analysis of applications in popular model developmental systems.

Main Results:

  • Genome engineering tools provide unprecedented precision for analyzing developmental processes.
  • These technologies are being applied across various model organisms to understand complex biological mechanisms.

Conclusions:

  • Designer genetic tools are revolutionizing developmental biology research.
  • The accessibility and robustness of these tools promise to expand studies into less-established experimental organisms.