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In Vitro Generation of Heart Field-specific Cardiac Progenitor Cells
Published on: July 3, 2019
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Arid3b is essential for second heart field cell deployment and heart patterning.
Verónica Uribe1, Claudio Badía-Careaga1, Jesús C Casanova1
1Departamento de Desarrollo y Reparación Cardiovascular, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernández Almagro, 3, Madrid 28029, Spain.
Development (Cambridge, England)
|October 23, 2014
Summary
The transcription factor Arid3b is crucial for heart development in mice. Its absence leads to cardiac abnormalities by affecting heart progenitor cell migration and differentiation.
Area of Science:
- Developmental biology
- Genetics
- Cardiovascular research
Background:
- The ARID family transcription factor Arid3b is vital for mouse embryonic development.
- Specific roles of Arid3b in cardiac development remain largely unelucidated.
Purpose of the Study:
- To investigate the function of Arid3b in heart development.
- To identify molecular mechanisms underlying Arid3b-mediated cardiac patterning.
Main Methods:
- Analysis of Arid3b expression in the developing heart.
- Phenotypic analysis of Arid3b-deficient mouse embryos.
- DiI labeling of second heart field progenitors.
- RNA microarray analysis.
Main Results:
- Arid3b is expressed in the myocardium and second heart field progenitors.
- Arid3b deficiency causes cardiac abnormalities, including shortened poles and absent myocardial differentiation.
- Defects in second heart field progenitor addition and epithelial-to-mesenchymal transition in the atrioventricular canal were observed.
- Arid3b regulates expression of genes like Bhlhb2 and Lims2, impacting progenitor cell migration and differentiation.
Conclusions:
- Arid3b is essential for regulating heart progenitor cell motility and differentiation during cardiac development.
- Arid3b plays a critical role in preventing congenital heart malformations.
Keywords:
AVC explantArid3bCell motilityDiI labellingHeart developmentMouseSecond heart fieldTranscription factorMore Related Videos
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