Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

826
The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic...
826
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

1.6K
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
1.6K
Mechanism of Conjugation01:19

Mechanism of Conjugation

1.5K
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
1.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

14.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
14.5K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

1.1K
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
1.1K
Sexually Transmitted Infections01:26

Sexually Transmitted Infections

1.8K
Sexually transmitted infections (STIs) are diseases transmitted primarily through unsafe sexual interactions. Bacteria, viruses, or parasites cause them and can result in severe health complications if untreated.ChlamydiaThe bacterium Chlamydia trachomatis is responsible for the disease Chlamydia, the most common STI in the United States. This peculiar pathogen requires human cells to reproduce, residing intracellularly. The initial infection often goes unnoticed because it typically does not...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aptameric Metal-Organic Framework Nanobiosensor.

ACS applied nano materials·2026
Same author

ATF3 Modulates the Endoplasmic Reticulum Stress-Induced Impairment of Milk Synthesis in Bovine Mammary Epithelial Cells.

International journal of molecular sciences·2026
Same author

Rapid and Non-Destructive Detection of Moisture Content in Dried Areca Nuts Based on Near-Infrared Spectroscopy Combined with Machine Learning.

Foods (Basel, Switzerland)·2026
Same author

Changes of Phenolic Profiles, Bioaccessibility, and Antioxidant Performance of <i>Areca catechu</i> L. Extracts During In Vitro Digestion.

Food science & nutrition·2026
Same author

Molecular characteristics of organic aerosols and impacts of transboundary biomass burning in Southwest China.

Journal of hazardous materials·2026
Same author

Epigenetically controlled endothelial promyelocytic leukemia drives liver inflammation and fibrosis.

The Journal of clinical investigation·2026

Related Experiment Video

Updated: Apr 21, 2026

Forward Genetic Approaches in Chlamydia trachomatis
09:03

Forward Genetic Approaches in Chlamydia trachomatis

Published on: October 23, 2013

12.4K

Conserved type III secretion system exerts important roles in Chlamydia trachomatis.

Wenting Dai1, Zhongyu Li1

  • 1Pathogenic Biology Institute, School of Medicine, University of South China Hengyang City, Hunan Province, P. R. China.

International Journal of Clinical and Experimental Pathology
|October 23, 2014
PubMed
Summary

Chlamydia trachomatis uses a conserved type III secretion system (T3SS) to manipulate host cells. This bacterial virulence factor delivers effector proteins, altering host functions like apoptosis and cytoskeleton organization.

Keywords:
Chlamydia trachomatischaperoneseffectorsinjectisomesregulatorstype III secretion system

More Related Videos

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
10:35

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Published on: January 30, 2020

6.4K
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
07:42

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Published on: October 13, 2015

6.9K

Related Experiment Videos

Last Updated: Apr 21, 2026

Forward Genetic Approaches in Chlamydia trachomatis
09:03

Forward Genetic Approaches in Chlamydia trachomatis

Published on: October 23, 2013

12.4K
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
10:35

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Published on: January 30, 2020

6.4K
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
07:42

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Published on: October 13, 2015

6.9K

Area of Science:

  • Microbiology
  • Cell Biology
  • Bacterial Pathogenesis

Background:

  • Chlamydiae are Gram-negative bacteria that significantly alter host cell functions during infection.
  • Key alterations include preventing apoptosis, reorganizing the actin cytoskeleton, and modifying host signaling pathways.
  • Pathogenic bacteria often utilize specialized secretion systems to deliver virulence factors into host cells.

Purpose of the Study:

  • To review the challenges and recent advances in understanding how Chlamydia trachomatis employs its type III secretion system (T3SS).
  • To highlight the role of T3SS diversification in producing a conserved system crucial for Chlamydia trachomatis virulence.

Main Methods:

  • This review synthesizes existing research on Chlamydia trachomatis and its T3SS.
  • It focuses on the mechanisms of effector protein delivery and host cell manipulation.
  • The review examines evolutionary aspects and recent discoveries regarding T3SS function.

Main Results:

  • Chlamydia trachomatis utilizes a conserved T3SS, often referred to as the injectisome, for virulence.
  • The T3SS delivers effector proteins into host cells, disrupting normal cellular processes.
  • Diversification strategies contribute to the conserved nature and effectiveness of the T3SS in Chlamydia trachomatis.

Conclusions:

  • The type III secretion system is a critical virulence determinant for Chlamydia trachomatis.
  • Understanding T3SS function provides insights into bacterial pathogenesis and host-pathogen interactions.
  • Continued research on T3SS mechanisms can reveal novel therapeutic targets.