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Inflammatory cells' role in acetic acid-induced colitis
Mohammad H Sanei1, Fatemeh Hadizadeh2, Peyman Adibi3
1Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Advanced Biomedical Research
|October 23, 2014
Summary
Neutrophils may not solely cause oxidative stress in colitis. This finding questions the effectiveness of corticosteroids for treating inflammatory bowel disease (IBD).
Area of Science:
- Gastroenterology
- Immunology
- Cell Biology
Background:
- Colitis pathogenesis involves free radicals from inflammatory cells and tissues.
- Corticosteroids can only target inflammatory cells, not tissue-derived factors.
- The role of neutrophils in colitis induction and corticosteroid efficacy requires further investigation.
Purpose of the Study:
- To assess the significance of neutrophils in inducing colitis.
- To evaluate the effectiveness of corticosteroids in treating inflammatory bowel disease (IBD).
Main Methods:
- Thirty-six mice were divided into six groups, with colitis induced by acetic acid enema or ex vivo exposure.
- Control groups received water instead of acetic acid.
- Macroscopic, biochemical (H2O2, FRAP), and pathological analyses were performed on tissue samples.
Main Results:
- Significant differences in neutrophilic infiltration, goblet cell depletion, and H2O2 levels were observed between experimental and control groups.
- Ferric Reducing Ability of Plasma (FRAP) showed significant variations across groups, indicating altered oxidative stress.
- Results suggest neutrophils are not the sole contributors to oxidative processes in colitis.
Conclusions:
- Neutrophils may not be the exclusive cause of oxidative stress in colitis.
- The efficacy of corticosteroids in treating colitis, particularly IBD, is rendered doubtful by these findings.
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