Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.

PLoS genetics·2014
Same author

Dynactin integrity depends upon direct binding of dynamitin to Arp1.

Molecular biology of the cell·2014
Same author

Quantitative proteomic profiling reveals differentially regulated proteins in cystic fibrosis cells.

Journal of proteome research·2014
Same author

Isolation of chromatin from dysfunctional telomeres reveals an important role for Ring1b in NHEJ-mediated chromosome fusions.

Cell reports·2014
Same author

Identification of small ubiquitin-like modifier substrates with diverse functions using the Xenopus egg extract system.

Molecular & cellular proteomics : MCP·2014
Same author

Mechanisms of acute kidney injury induced by experimental Lonomia obliqua envenomation.

Archives of toxicology·2014

Related Experiment Video

Updated: Apr 21, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

4.9K

Isobaric labeling-based relative quantification in shotgun proteomics.

Navin Rauniyar1, John R Yates

  • 1Department of Chemical Physiology, The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

Journal of Proteome Research
|October 23, 2014
PubMed
Summary
This summary is machine-generated.

Isobaric labeling in mass spectrometry improves protein quantification accuracy. This review details reagents, chemistry, and methods to overcome challenges like ratio compression for better proteomic analysis.

Keywords:
TMTiTRAQisobaric labelingisobaric tagsisobaric tags for relative and absolute quantificationmass spectrometryquantitative proteomicstandem mass tags

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.4K
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

5.4K

Related Experiment Videos

Last Updated: Apr 21, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

4.9K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.4K
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

5.4K

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Mass spectrometry is crucial for relative protein quantification in biological systems.
  • Isobaric labeling techniques are widely used in shotgun proteomic analysis.

Purpose of the Study:

  • To review studies on isobaric labeling-based relative quantification in shotgun proteomics.
  • To identify factors affecting quantification accuracy and propose solutions.
  • To examine extended applications of isobaric labeling.

Main Methods:

  • Focus on various isobaric reagents and their reaction chemistry (amine-, carbonyl-, sulfhydryl-reactive).
  • Analysis of factors causing underestimation of protein abundance changes (e.g., ratio compression, reporter ion dynamic range).
  • Discussion of experimental design and data acquisition strategies for improved precision and accuracy.

Main Results:

  • Identified key factors that limit the quantitative accuracy of isobaric labeling.
  • Highlighted strategies to enhance measurement precision and accuracy.
  • Examined advanced applications including hyperplexing, targeted quantification, and phosphopeptide analysis.

Conclusions:

  • Optimizing experimental design and data acquisition is essential for accurate isobaric labeling-based proteomics.
  • Isobaric labeling offers versatile applications beyond basic relative quantification.