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Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine
Aymeric Hans1, Delphine Gaudaire2, Jean-Claude Manuguerra3
1ANSES-Dozulé Laboratory for Equine Diseases, Virology Unit, Goustranville, France aymeric.hans@anses.fr.
Journal of Clinical Microbiology
|October 24, 2014
Abstract:
This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.
