[SDS electrophoretic evaluation of salivary protein adsorption to different dental materials]

After different pretreatments of surface the alloys Sipal, Gisadent KCM 83 and Gisadent NCA and the PMME resin Kallocryl A were contaminated with mixed saliva for 1.5 hours. Thereafter, the proteins which could not washed off with water were desorbed from the materials by using a dodecyl sulphate-containing buffer, and analyzed SDS electrophoretically and densitometrically. The pattern of the proteins desorbed reflected a fairly unspecific adsorption of the salivary proteins independent on the kind of the dental material used. Whereas the ranges of the low (10-25 kDa) and the high (greater than 50 kDa) molecular mass polypeptides share nearly equal quotas in the protein pattern of the original saliva, a generally higher portion (68-99%) of the smaller polypeptides was shown among the proteins desorbed from the materials. This effect was especially marked after a shot-peening of alloys and a very high polishing of resin. A somewhat higher percentage of the larger proteins (18-51%) were desorbed from the materials which were pretreated by fine-smoothing or sandblasting.