Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

17.3K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
17.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laparoscopic extraperitoneal techniques in ventral hernia repair: a retrospective comparative study of TAPP and TEP.

Journal of abdominal wall surgery : JAWS·2026
Same author

Author Correction: An asymmetric fission island driven by shell effects in light fragments.

Nature·2025
Same author

An asymmetric fission island driven by shell effects in light fragments.

Nature·2025
Same author

A brief history of visualizing membrane systems in molecular dynamics simulations.

Frontiers in bioinformatics·2023
Same author

[Ophthalmic follow-up of patients treated with antimalarial drugs: Evaluation of patient attitudes regarding the change in protocol].

Journal francais d'ophtalmologie·2021
Same author

Evidence for a New Compact Symmetric Fission Mode in Light Thorium Isotopes.

Physical review letters·2020

Related Experiment Video

Updated: Apr 21, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

8.2K

Innovative interactive flexible docking method for multi-scale reconstruction elucidates dystrophin molecular

A-E Molza1, N Férey, M Czjzek

  • 1Institut de Génétique et Développement de Rennes, CNRS, UMR6290, Univ. Rennes 1, Campus Santé, 2 av du Pr Léon Bernard, 35043 Rennes Cedex, France. olivier.delalande@univ-rennes1.fr.

Faraday Discussions
|October 24, 2014
PubMed
Summary

Researchers developed a new interactive method combining BioSpring software and SAXS data to model dystrophin, a protein linked to myopathy. This approach provides the first high-resolution models of dystrophin

More Related Videos

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

15.3K
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

10.5K

Related Experiment Videos

Last Updated: Apr 21, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

8.2K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

15.3K
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

10.5K

Area of Science:

  • Biophysics
  • Structural Biology
  • Computational Biology

Background:

  • Molecular knowledge of dystrophin, crucial for muscle function and affected in Duchenne muscular dystrophy (DMD), is limited due to the lack of its atomic structure.
  • Understanding dystrophin's structure and interactions is vital for developing therapies for myopathies.

Purpose of the Study:

  • To develop an innovative computational method for modeling dystrophin structure and interactions.
  • To generate high-resolution models of the central filamentous domain of dystrophin.
  • To investigate the potential interactions of dystrophin with F-actin and neuronal nitric oxide synthase (nNOS).

Main Methods:

  • Developed an interactive docking method using BioSpring software, incorporating an augmented Elastic Network Model (aENM).
  • Combined atomistic and coarse-grained models for multi-scale simulations, optimized with parallel computing and GPU programming.
  • Integrated Small-angle X-ray Scattering (SAXS) data as restraints for molecular modeling.

Main Results:

  • Generated the first high-resolution models of the filamentous central domain of dystrophin (repeats 11-17).
  • Proposed potential models for dystrophin association with F-actin and nNOS through low-resolution interactive docking.
  • Demonstrated the efficiency of the BioSpring-SAXS approach for biomolecular model design and simulation preparation.

Conclusions:

  • The developed interactive docking method effectively models complex biomolecules like dystrophin even without atomic structures.
  • The generated dystrophin models and proposed interactions offer new insights into muscle function and disease mechanisms.
  • This approach holds promise for future discoveries in myopathy research and therapeutic development.