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Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
Published on: December 14, 2015
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Development of a terminally sterilised decellularised dermis
P Hogg1, P Rooney, S Leow-Dyke
1NHSBT, Tissue Services R&D, 14 Estuary Banks, Speke, Liverpool, L24 8RB, UK, penny.hogg@nhsbt.nhs.uk.
Cell and Tissue Banking
|October 25, 2014
Summary
Terminal sterilization using gamma irradiation effectively produces dCELL human dermis, a decellularized allograft. This method ensures tissue integrity and biocompatibility for transplantation, showing successful integration in vivo.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Current decellularized dermis products are often aseptically produced.
- NHS Blood and Transplant Tissue Services developed a novel production method for dCELL human dermis.
Purpose of the Study:
- To evaluate the safety and efficacy of terminally sterilized decellularized human dermis (dCELL).
- To compare gamma-irradiated dCELL with native cellular tissue.
Main Methods:
- Histological examination, DNA content analysis, biomechanical and biochemical property testing.
- In vitro cytotoxicity assays and in vivo implantation studies in a mouse model.
Main Results:
- No significant morphological alterations observed via light microscopy.
- 99% DNA removal achieved with no significant changes in ultimate tensile stress or collagen denaturation.
- No cytotoxicity or adverse in vivo tissue reactions noted; successful host incorporation demonstrated.
Conclusions:
- Decellularization followed by terminal gamma irradiation is a suitable method for producing human dermis allografts.
- Terminally sterilized dCELL human dermis is appropriate for transplantation.
- The process preserves tissue properties and ensures biocompatibility.
