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Updated: Apr 21, 2026
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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
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Full-Genome Assembly of Reference Strain Providencia stuartii ATCC 33672
K G Frey, K A Bishop-Lilly, H E Daligault1
1Los Alamos National Laboratory (LANL), Los Alamos, New Mexico, USA.
Genome Announcements
|October 25, 2014
Summary
We sequenced the complete genome of Providencia stuartii, a bacterium linked to hospital-acquired infections and antibiotic resistance. This provides a foundation for understanding its clinical significance and resistance mechanisms.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Providencia stuartii is a normal human gut microflora member.
- It is clinically significant due to nosocomial infections, particularly urinary tract infections.
- This bacterium is known for its rapid acquisition of antibiotic resistance.
Purpose of the Study:
- To present the complete genome sequence of Providencia stuartii strain ATCC 33672.
- To provide a genomic resource for studying the bacterium's clinical relevance and antibiotic resistance.
Main Methods:
- Whole-genome sequencing of Providencia stuartii strain ATCC 33672.
- Bioinformatic analysis of the resulting genome data.
Main Results:
- The complete genome of P. stuartii ATCC 33672 comprises a 4.28-Mbp chromosome.
- A 48.9-kbp plasmid was also identified and sequenced.
- The genome sequence provides a comprehensive genetic blueprint of the strain.
Conclusions:
- The complete genome sequence of P. stuartii ATCC 33672 is now available.
- This genomic data will aid future research into its pathogenic mechanisms and antibiotic resistance.
- Facilitates the development of targeted interventions against P. stuartii infections.
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