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Published on: December 26, 2019
Effects of hemostatic agents on fibroblast cells
Yusuf Emes1, Buket Aybar, Pervin Vural
1*Associate Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul University, Istanbul, Turkey. †Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul University, Istanbul, Turkey. ‡Professor, Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul University, Istanbul, Turkey. §Professor, Istanbul Faculty of Medicine, Department of Public Health, Istanbul University, Istanbul, Turkey. ‖Professor, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul University, Istanbul, Turkey. ¶Associate Professor, Istanbul University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul, Turkey. #Research Assistant, Istanbul University, Faculty of Dentistry, Department of Endodontics Istanbul, Turkey. **Professor, Istanbul University, Istanbul Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey.
Purpose:
Hemostatic agents may be used topically to control hemorrhage, especially in patients with bleeding disorders. The agent used may have a negative effect on the tissue prolonging the healing time. The aim of this study was to compare the effects of 3 different hemostatic agents on fibroblast cells on a rat primary fibroblast cell culture model.
Materials And Methods:
Ankaferd Blood Stopper (ABD) (Ankaferd Pharmaceuticals Cosmetics Production and Marketing Co.), fibrin glue, and tranexamic acid were the agents to be evaluated for their effects on cell proliferation, cell numbers, cell viability, and cell morphology. Also lactate dehydrogenase, basic fibroblast growth factor, and vascular endothelial growth factor C levels were measured.
Results:
It was found that all of the agents used in the study have negative effects on fibroblasts, with ABD having the lowest values of cell proliferation, cell number, and cell viability.
Conclusion:
The results of this study indicate that ABD, fibrin glue, and tranexamic acid may negatively affect tissue healing.
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