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Related Experiment Video

Updated: Apr 21, 2026

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
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Single cell adhesion assay using computer controlled micropipette.

Rita Salánki1, Csaba Hős2, Norbert Orgovan3

  • 1Doctoral School of Molecular- and Nanotechnologies, University of Pannonia, Veszprém, Hungary; Nanobiosensorics Group, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Budapest, Hungary; Department of Biological Physics, Eötvös University, Budapest, Hungary.

Plos One
|October 25, 2014
PubMed
Summary
This summary is machine-generated.

A new computer-controlled micropipette system enables rapid, high-throughput measurement of single-cell adhesion forces to macromolecules. This technology reveals that dendritic cells exhibit the strongest adhesion to fibrinogen, surpassing monocytes and macrophages.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Immunology

Background:

  • Cell adhesion is critical for multicellular life, particularly for leukocytes initiating immune responses.
  • Measuring single-cell adhesion forces is challenging, with current methods offering very low throughput.

Purpose of the Study:

  • To develop a high-throughput method for measuring single-cell adhesion forces to specific macromolecules.
  • To investigate differences in fibrinogen adhesion among human monocytes, macrophages, and dendritic cells.

Main Methods:

  • A computer-controlled micropipette system was developed for probing single-cell interactions.
  • Hydrodynamic lifting force was calculated via numerical flow simulation.
  • Adhesion force was measured by increasing vacuum in the micropipette.
  • Non-specific adhesion was blocked using PLL-g-PEG.

Main Results:

  • The new method achieved high throughput, measuring hundreds of cells in approximately 30 minutes.
  • Human primary monocytes showed lower adhesion to fibrinogen compared to macrophages and dendritic cells.
  • Dendritic cells exhibited the highest average adhesion force to fibrinogen, with a sub-population of strongly adherent cells.

Conclusions:

  • The computer-controlled micropipette is a sensitive, high-throughput tool for cell adhesion force measurements.
  • Differentiated immune cells (macrophages, dendritic cells) display distinct and increased fibrinogen adhesion compared to monocytes.
  • A sub-population of strongly adherent cells exists, particularly in dendritic cells.