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Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
Cellular composition of periapical granulomas and its function. Histological, immunohistochemical and
P Babál1, M Brozman, J Jakubovský
1School of Medicine, Comenius University, Bratislava.
Czechoslovak Medicine
|January 1, 1989
Summary
Periapical granulomas involve inflammatory tissue with numerous plasma cells producing immunoglobulins. Immune complexes likely contribute to pathogenesis, but typical cell-mediated immunity is not suggested.
Area of Science:
- Oral Pathology
- Immunology
- Histopathology
Background:
- Periapical granulomas are inflammatory lesions at the root apex of teeth.
- Understanding their cellular and immunological components is crucial for diagnosis and treatment.
Purpose of the Study:
- To investigate the histological, immunohistological, and ultrastructural features of periapical granulomas.
- To elucidate the role of immune responses in the pathogenesis of these lesions.
Main Methods:
- Histological examination of lesion tissues.
- Immunohistochemical analysis using antibodies for immunoglobulins (IgG, IgA, IgM, IgE, IgD) and complement components (C3, C4).
- Electron microscopy to study cellular ultrastructure.
Main Results:
- Lesions showed inflammatory granulation tissue with plasma cells, lymphocytes, and fibroblasts.
- Plasma cells predominantly produced IgG.
- T-suppressor/cytotoxic lymphocytes outnumbered T-helper lymphocytes.
- Immune complex components (C3, C4) were detected.
- Mast cells were numerous but not degranulated.
Conclusions:
- Immune complexes likely play a role in periapical granuloma pathogenesis.
- Anaphylactic reactions are unlikely.
- The prevalence of T-suppressor/cytotoxic lymphocytes and low macrophage numbers may contribute to chronicity.
