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Effects of actin-like proteins encoded by two Bacillus pumilus phages on unstable lysogeny, revealed by genomic

Yihui Yuan1, Qin Peng1, Dandan Wu1

  • 1Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, People's Republic of China.

Applied and Environmental Microbiology
|October 26, 2014
PubMed

Insights

Two bacteriophages, Bp8p-C and Bp8p-T, were identified that infect Bacillus pumilus GR8, the cause of ginger rhizome rot. Bp8p-C demonstrated superior biocontrol efficacy against this pathogen in ginger slices.

Area of Science:

  • Microbiology
  • Virology
  • Biotechnology

Background:

  • Ginger rhizome rot disease, caused by Bacillus pumilus GR8, poses a significant threat to ginger cultivation.
  • Bacteriophages are viruses that infect bacteria and show potential for biological control of plant pathogens.

Purpose of the Study:

  • To characterize two newly isolated myoviruses, Bp8p-C and Bp8p-T, that infect Bacillus pumilus GR8.
  • To evaluate their potential for biocontrol of ginger rhizome rot disease.

Main Methods:

  • Isolation and characterization of bacteriophages Bp8p-C and Bp8p-T.
  • Genome sequencing and comparative analysis.
  • Lysogeny assays.
  • Functional analysis of phage proteins (Gp27).
  • Structural proteome analysis of Bp8p-C virion.
  • In vitro biocontrol assays using ginger rhizome slices.

Main Results:

  • Both Bp8p-C and Bp8p-T are myoviruses infecting Bacillus pumilus GR8 and can form latent states (lysogeny), with Bp8p-T showing higher frequency and stability.
  • Genomic analysis revealed minimal differences between the phages, primarily in the Gp27 gene, affecting protein function and lysogenic ability.
  • Structural proteome analysis identified 14 proteins in the Bp8p-C virion, including novel components like pectin lyase-like protein and poly-gamma-glutamate hydrolase.
  • Bp8p-C exhibited a better control effect on Bacillus pumilus GR8 in ginger rhizome slices compared to Bp8p-T.

Conclusions:

  • The identified bacteriophages, particularly Bp8p-C, hold promise for the biological control of ginger rhizome rot.
  • Differences in the Gp27 protein significantly influence the lysogenic capabilities of these phages.
  • Novel structural proteins were identified in the Bp8p-C virion, expanding our understanding of phage biology.

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