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Updated: Apr 21, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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[Recent advance in high accuracy iTRAQ for quantitative proteomics].

Shouzhi Ma, Yulin Sun, Xiaohang Zhao

    Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
    |October 28, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Isobaric tags for relative and absolute quantitation (iTRAQ) is a key technique for quantitative proteomics, offering high throughput and stability across diverse samples. This review highlights iTRAQ advancements and its broad applications in biological research.

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    Area of Science:

    • Proteomics
    • Mass Spectrometry
    • Biotechnology

    Background:

    • Quantitative proteomics is increasingly important, shifting focus from qualitative analysis.
    • Isobaric tags for relative and absolute quantitation (iTRAQ) is a widely adopted technique in this field.
    • iTRAQ offers advantages like high throughput, stability, and broad sample applicability.

    Purpose of the Study:

    • To review recent advancements in iTRAQ technology.
    • To summarize the diverse applications of iTRAQ in quantitative proteomics.
    • To highlight the integration of iTRAQ with various mass spectrometry platforms.

    Main Methods:

    • iTRAQ labeling for multiplexed quantitative proteome analysis.
    • Mass spectrometry (MS) for peptide identification and quantification.
    • Review of published literature on iTRAQ development and applications.

    Main Results:

    • iTRAQ enables simultaneous analysis of up to 8 samples.
    • Improved accuracy of iTRAQ with advancements in MS platforms.
    • Successful application of iTRAQ across various fields including microbiology, medicine, and plant science.

    Conclusions:

    • iTRAQ is a versatile and powerful tool for quantitative proteomics.
    • Ongoing technological developments continue to enhance iTRAQ's capabilities.
    • iTRAQ plays a crucial role in diverse biological and medical research areas.