[Optimization of fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana]

To optimize the fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana.

The medium composition and cultivation conditions were optimized by orthogonal and single factor experiments.

The experiments showed that the conditions of initial pH 3, fermentation temperature 30 degrees C, volume of liquid 100 ml (250 ml Erlenmeyer flask), and 3D-xylitol 0.5 g/L were optimum, and the molluscicidal activity of the fermentation filtrate reached 95%. After three hatches of cultivation, the predicted values were verified by validation experiments.

Endophyte LL3026 from Buddleia lindleyana has a good molluscicidal activity after the optimization.