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Updated: Apr 21, 2026
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Synergistic coupling of fluorescent "turn-off" with spectral overlap modulated FRET for ratiometric Ag+ sensor
Mengliang Zhu1, Yabin Zhou, Liguo Yang
1Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, Department of Chemistry, University of Science and Technology Beijing , Beijing 100083, China.
Abstract:
A useful strategy for ratiometric fluorescent detecting of Ag(+) is demonstrated. Upon selective binding of Ag(+) to a BODIPY-porphyrin dyad (1), the synergistic coupling of two functions, namely the suppressing of FRET from BODIPY donor to porphyrin acceptor and the fluorescence quenching of porphyrin acceptor, leads to exceptionally large changes in the intensity ratio of two distinct emissions (F513/F654) which allow for the ratiometric detecting of Ag(+) with excellent sensitivity in solution and living cells.
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