Low molecular weight fibrinolytic inhibitor from Guerin epithelioma

Antifibrinolytic activity of the extract from Guerin epithelioma, a highly metastatic tumour implanted to rats, was determined by fibrinolytic and zymographic methods. The extract exhibits antifibrinolytic activity which is thermostable (60-100 degrees C) and pH-stable (pH 2.7-12). It contains a fibrinolytic inhibitor, with Mr about 7000, with antiplasmin properties, bound to lys-Sepharose and heparin-Sepharose. The molecular weight, physicochemical properties and antiplasmin action of the epithelioma inhibitor prove its identity with the low molecular weight antifibrinolytic factor appearing in the plasma of rats during the development of this tumour.