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Updated: Aug 12, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
[Analysis of (+/-) anisodamine by means of HPLC]
Abstract:
A study on the quantitative-determination of a synthetic new drug--(+/-) anisodamine with HPLC is described. The analysis was completed on a stainless steel column (phi 4 X 250 mm) packed with a chemical bonded phase (YWG-C18H37), the mobile phase being a mixture of methanol, water and triethylamine (V/V/V 255:45:0.01) with a flow rate of 1 ml/min at room temperature. The (+/-)-anisodamine was detected by UV at 254 nm. This method is rapid, reproducible and accurate, and can be used for quantitative determination of (+/-) anisodamine in different product batches.
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