[Isolation and characterization of fibronectin from porcine plasma]

Electrophoretic purified fibronectin (FN) was isolated with high concentration (0.5 mg/ml plasma) from porcine plasma by affinity chromatography with gelatin-sepharose 4B and heparin-sepharose 4B. The isolated FN shows one single band (MW 450,000) both in agarose gel and PAGE, and two similar bands (MW 230,000) in the presence of 1% beta-mercaptoethanol in SDS-PAGE. The FN isolated remains its immunological and biological properties, being reactable with antibodies against human and bovine FN with strong promotion effect on CHO cell adhesion in serum free medium. These data indicate that porcine plasma is a good resource for isolation of FN.