Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Study on rapid serodiagnosis for leprosy].

Q X Wu

    Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
    |October 1, 1989
    PubMed
    Summary

    Two new latex agglutination reaction (LAR) tests for leprosy diagnosis show sensitivity and specificity comparable to ELISA. These simpler, rapid LAR tests offer potential for field-based immunodiagnosis of leprosy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Study on mosaicism of SCN1A gene mutation in parents of children with Dravet syndrome].

    Zhonghua er ke za zhi = Chinese journal of pediatrics·2017
    Same author

    Unraveling the Numerous Biosynthetic Products of the Marine Sediment-Derived Fungus, Aspergillus insulicola.

    Phytochemistry letters·2012
    Same author

    Nontuberculous mycobacterial cutaneous infection confirmed by biochemical tests, polymerase chain reaction-restriction fragment length polymorphism analysis and sequencing of hsp65 gene.

    The British journal of dermatology·2003
    Same author

    Fatal anaphylaxis following jack jumper ant sting in southern Tasmania.

    The Medical journal of Australia·2002
    Same author

    Phylogenetic and biogeographic relationships of eastern Asian and eastern North American disjunct Suillus species (fungi) as inferred from nuclear ribosomal RNA ITS sequences.

    Molecular phylogenetics and evolution·2000
    Same author

    Cytotoxicity of pilosulin 1, a peptide from the venom of the jumper ant Myrmecia pilosula.

    Biochimica et biophysica acta·1998

    Area of Science:

    • Immunology
    • Medical Diagnostics
    • Microbiology

    Background:

    • Leprosy diagnosis relies on clinical and laboratory methods.
    • Existing serological tests like ELISA can be complex and time-consuming.
    • Need for rapid, field-deployable diagnostic tools for leprosy.

    Purpose of the Study:

    • To develop and evaluate two latex agglutination reaction (LAR) tests for leprosy immunodiagnosis.
    • To compare the performance of PGL-1-LAR and ND-O-BSA-LAR with established PGL-1-ELISA and ND-O-BSA-ELISA.
    • To assess the utility of LAR tests for leprosy classification and field application.

    Main Methods:

    • Development of two LAR tests using PGL-1 and ND-O-BSA antigens.
    • Testing of 80 leprosy patients across different clinical types (LL, BL, BT, TT), 20 tuberculosis cases, and 25 normal controls.
    • Comparative analysis of LAR tests against corresponding ELISAs using Chi-square tests.

    Main Results:

    • Both PGL-1-LAR and ND-O-BSA-LAR demonstrated sensitivity and specificity comparable to their respective ELISAs.
    • No significant statistical difference was found between LAR and ELISA performance (P > 0.10 for PGL-1-LAR vs. ND-O-BSA-LAR, P > 0.10 for PGL-1-LAR vs. PGL-1-ELISA, P > 0.25 for ND-O-BSA-LAR vs. ND-O-BSA-ELISA).
    • Positive rates correlated with the clinical classification of leprosy.

    Conclusions:

    • Latex agglutination reaction tests (LAR) are effective immunodiagnostic tools for leprosy.
    • LAR tests offer advantages of simplicity and speed over ELISA, making them suitable for field use.
    • Further research should focus on optimizing conditions for stability and reproducibility of LAR tests in diverse settings.

    Related Experiment Videos