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Updated: Apr 21, 2026
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
Published on: May 10, 2015
Measurement of deubiquitinating enzyme activity via a suicidal HA-Ub-VS probe
Colleen Rivard1, Martina Bazzaro
1Department of Obstetrics, University of Minnesota Medical School, MMC 395 Mayo, 8395C (Campus Delivery Code), 420 Delaware Street SE, Minneapolis, MN, 55455, USA, clrivard@umn.edu.
Abstract:
Deubiquitinating enzymes (DUBs) are a novel "drug-able" target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.
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