Lysinibacillus fluoroglycofenilyticus sp. nov., a bacterium isolated from fluoroglycofen contaminated soil

A novel Gram-positive, fluoroglycofen-degrading bacterium, designated cmg86(T), was isolated from herbicide contaminated soil collected from Tongjing, Jiangsu province, China. Strain cmg86(T) was found to be aerobic, motile, endospore-forming rods. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain cmg86(T) belongs to the genus Lysinibacillus and showed the highest sequence similarity to Lysinibacillus meyeri DSM 25057(T) (97.9 %) and Lysinibacillus odysseyi KCTC 3961(T) (96.6 %). The cell-wall peptidoglycan type was determined to be A4α (L-Lys-D-Asp), which is consistent with the cell-wall characteristics of the genus Lysinibacillus. The predominant respiratory quinones were identified as menaquinone-7 (MK-7, 89.5 %) and meanaquinone-6 (MK-6, 8.9 %), and the major fatty acids were identified as iso-C15:0, anteiso-C15:0 and antesio-C17:0. The major polar lipids were found to be phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content of strain cmg86(T) was determined to be 37.6 mol%. The results of this study support the conclusion that strain cmg86(T) represents a novel species of the genus Lysinibacillus for which the name and Lysinibacillus fluoroglycofenilyticus sp. nov. is proposed. The type strain is cmg86(T) (=KCTC 33183(T) = CCTCC AB 2013247(T)).