Related Experiment Video

Updated: Apr 21, 2026

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
13:39

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

18.9K

Mycoplasma Contamination of Cell Cultures: Vesicular Traffic in Bacteria and Control over Infectious Agents

V M Chernov1, O A Chernova1, J T Sanchez-Vega2

  • 1Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Lobachevskogo Str., 2/3, 1420111, Kazan, Russia ; Kazan (Volga Region) Federal University, Kremlyovskaya Str., 18, 420008, Kazan, Russia.

Acta Naturae
|October 29, 2014
PubMed

Insights

Mycoplasma contamination threatens cell cultures, impacting vaccine production. Understanding bacterial extracellular vesicles is key to developing new diagnostic and eradication strategies for these persistent contaminants.

Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Cell cultures are vital for research, vaccine production, and biopharmaceutical manufacturing.
  • Mycoplasma contamination poses a significant threat to cell culture integrity and experimental validity.
  • Bacterial extracellular vesicles play roles in pathogenesis and antibiotic resistance.

Purpose of the Study:

  • To review the characteristics of mycoplasmas and their extracellular vesicles.
  • To examine the interaction between mycoplasma contaminants and eukaryotic cells.
  • To analyze current challenges and future solutions for mycoplasma contamination in cell cultures.

Main Methods:

  • Literature review of mycoplasma biology and extracellular vesicles.
  • Analysis of diagnostic and eradication methods for mycoplasma contamination.
  • Discussion of host-pathogen interactions involving mycoplasmas.

Main Results:

  • Mycoplasmas utilize extracellular vesicles for intercellular communication and pathogenesis.
  • Existing diagnostic and eradication methods for mycoplasma contamination have limitations.
  • Bacterial vesicular traffic must be considered in contaminant control systems.

Conclusions:

  • Effective control of mycoplasma contamination requires novel approaches considering bacterial vesicle function.
  • Further research into mycoplasma extracellular vesicles can lead to improved detection and elimination strategies.
  • Addressing mycoplasma contamination is crucial for reliable biotechnological production and scientific research.
Keywords:
cell culturesdiagnosis and eradicationmycoplasma contamination

More Related Videos

Optimized PCR-based Detection of Mycoplasma06:01

Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

56.1K
Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria06:38

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

1.8K

Related Concept Videos

Key Techniques in Microbiology01:19

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
3.0K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
1.4K
Need for Obtaining Pure Cultures01:29

Need for Obtaining Pure Cultures

Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
2.6K
Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
2.7K