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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
High Incidence of Staphylococcus aureus and Norovirus Gastroenteritis in Infancy: A Single-Center, 1-Year Experience
Kyoung Sung1, Ji Yong Kim1, Yeoun Joo Lee1
1Department of Pediatrics, Pusan National University Children's Hospital, Yangsan, Korea.
Insights
Staphylococcus aureus and norovirus are the leading causes of acute gastroenteritis in infants following rotavirus vaccination. This study identified common bacterial and viral pathogens contributing to infant AGE.
Area of Science:
- Pediatrics
- Infectious Diseases
- Microbiology
Background:
- Rotavirus vaccination has altered the landscape of acute gastroenteritis (AGE) etiology in infants.
- Understanding current common pathogens is crucial for effective diagnosis and treatment.
Purpose of the Study:
- To identify the prevalent bacterial and viral pathogens causing acute gastroenteritis in infants.
- To assess changes in AGE etiology post-rotavirus vaccination.
Main Methods:
- Stool specimens from infants with acute diarrhea were tested for 10 bacterial and 5 viral pathogens.
- Clinical symptoms and laboratory data were recorded and compared between bacterial and viral cases.
Main Results:
- 51.2% of infants tested positive for at least one pathogen.
- Staphylococcus aureus (31.7%) and norovirus (12.2%) were the most common bacterial and viral pathogens, respectively.
- Fever and respiratory symptoms were more frequent in viral infection cases.
Conclusions:
- Staphylococcus aureus and norovirus are currently the primary bacterial and viral agents of AGE in infants.
- The findings highlight the evolving nature of infectious diarrhea in infants.
Purpose:
The etiology of acute gastroenteritis (AGE) has changed since the introduction of the rotavirus vaccination. The aim of this study was to clarify which common pathogens, both bacterial and viral, are currently causing AGE in infants.
Methods:
Infants with acute diarrhea were enrolled. We tested for 10 bacterial pathogens and five viral pathogens in stool specimens collected from infants with AGE. The clinical symptoms such as vomiting, mucoid or bloody diarrhea, dehydration, irritability, and poor oral intake were recorded, and laboratory data such as white blood cell count and C-reactive protein were collected. The clinical and laboratory data for the cases with bacterial pathogens and the cases with viral pathogens were compared.
Results:
Of 41 total infants, 21 (51.2%) were positive for at least one pathogen. Seventeen cases (41.5%) were positive for bacterial pathogens and seven cases (17.1%) were positive for viral pathogens. Staphylococcus aureus (13 cases, 31.7%) and Clostridium perfringens (four cases, 9.8%) were common bacterial pathogens. Norovirus (five cases, 12.2%) was the most common viral pathogen. Fever and respiratory symptoms were common in the isolated viral infection group (p=0.023 and 0.044, respectively), whereas other clinical and laboratory data were indistinguishable between the groups.
Conclusion:
In our study, S. aureus (41.5%) and norovirus (12.2%) were the most common bacterial and viral pathogens, respectively, among infants with AGE.
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