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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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The technique...
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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
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Decoding neural transcriptomes and epigenomes via high-throughput sequencing.

Jaehoon Shin1, Guo-li Ming2, Hongjun Song2

  • 11] Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. [2] Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Nature Neuroscience
|October 29, 2014
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) offers powerful tools for neuroscience research. This technology deciphers neuronal molecular signatures and epigenetic changes, advancing our understanding of brain function and maintenance.

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Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • The mammalian brain's complexity relies on dynamic cellular states for information processing and maintenance.
  • Understanding how neurons modify and retain molecular signatures is crucial for neuroscience.
  • Next-generation sequencing (NGS) is revolutionizing the study of neuronal molecular mechanisms.

Purpose of the Study:

  • To demystify Next-generation sequencing (NGS) technology for the neuroscience field.
  • To highlight the applications and potential of NGS in understanding neuronal function.
  • To provide practical information on utilizing NGS for epigenetic and transcriptome analyses.

Main Methods:

  • Discussion of the nervous system's complexity.
  • Introduction to various NGS applications and practical considerations.
  • Explanation of the basic principles underlying different NGS technologies.

Main Results:

  • NGS enables comprehensive transcriptome analyses to define cellular molecular signatures.
  • NGS facilitates epigenetic analyses to understand the cascade of events altering cellular states.
  • The study provides a foundational overview of NGS for neuroscience researchers.

Conclusions:

  • NGS technology is a transformative tool for probing the molecular basis of neuronal function.
  • Understanding NGS is essential for advancing neuroscience research in cellular states and molecular signatures.
  • Emerging NGS-related technologies hold significant promise for future neuroscience discoveries.