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Updated: Apr 21, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
TMIO-PyrImid hybrids are profluorescent, site-directed spin labels for nucleic acids
Benjamin A Chalmers1, Subham Saha, Tri Nguyen
1Faculty of Science and Engineering, Queensland University of Technology , P.O. Box 2434, 2 George Street, Brisbane, QLD 4001, Australia.
Abstract:
We report the synthesis of a new class of molecules which are hybrids of long-lived tetramethylisoindolinoxyl (TMIO) radicals and the pyrido[1,2-a]benzimidazole (PyrImid) scaffold. These compounds represent a new lead for noncovalently binding nucleic acid probes, as they interact with nucleic acids with previously unreported C (DNA) and C/U (RNA) complementarity, which can be detected by electron paramagnetic resonance (EPR) techniques. They also have promising properties for fluorimetric analysis, as their fluorescent spin-quenched derivatives exhibit a significant Stokes shift.

