Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Best Practices of Hind Limb Transplantation in Mice.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2026
Same author

Renal Function Decline After Vascularized Composite Allotransplantation.

Transplantation direct·2026
Same author

Implementation of a controlled DCD program in Western Austria - key considerations and insights.

Transplant international : official journal of the European Society for Organ Transplantation·2026
Same author

The integration of psychosocial care, ethical governance, and patient-centered research in current and future approaches in the field of vascularized composite allotransplantation.

Frontiers in transplantation·2026
Same author

Correction: Effect of normothermic machine perfusion on glycocalyx shedding during liver transplantation - a prospective pilot study.

Transplant international : official journal of the European Society for Organ Transplantation·2026
Same author

Author Correction: Injured epithelial cell states impact kidney allograft survival after T-cell-mediated rejection.

Nature communications·2026

Related Experiment Video

Updated: Apr 21, 2026

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
10:32

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Published on: October 12, 2014

13.9K

Murine cervical heart transplantation model using a modified cuff technique.

Rupert Oberhuber1, Benno Cardini1, Markus Kofler1

  • 1Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Innsbruck Medical University.

Journal of Visualized Experiments : Jove
|October 29, 2014
PubMed
Summary

A novel non-suture cuff technique enables successful cervical heart transplantation in mice. This microsurgical method achieves high patency rates, facilitating in vivo mechanistic studies with mouse models.

More Related Videos

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
06:45

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Published on: February 7, 2022

3.5K
Heterotopic Cervical Heart Transplantation in Mice
09:48

Heterotopic Cervical Heart Transplantation in Mice

Published on: August 25, 2015

11.2K

Related Experiment Videos

Last Updated: Apr 21, 2026

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
10:32

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Published on: October 12, 2014

13.9K
A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
06:45

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Published on: February 7, 2022

3.5K
Heterotopic Cervical Heart Transplantation in Mice
09:48

Heterotopic Cervical Heart Transplantation in Mice

Published on: August 25, 2015

11.2K

Area of Science:

  • * Transplantation research
  • * In vivo animal models
  • * Microsurgery

Background:

  • * Mouse models are crucial for in vivo mechanistic studies due to available resources.
  • * Traditional suture techniques for rat heart transplantation are technically complex for mice.
  • * Non-suture cuff techniques, adapted from rats, offer a viable alternative for murine models.

Purpose of the Study:

  • * To detail a refined non-suture cuff technique for cervical heart transplantation in mice.
  • * To establish a reliable and reproducible murine model for transplantation research.

Main Methods:

  • * Adaptation of a non-suture cuff technique for vascular anastomosis.
  • * Key steps involve everting recipient vessels over a cuff and placing the donor vessel over it.
  • * Anastomosis of the common carotid artery/aortic arch for arterial inflow and the pulmonary artery/jugular vein for venous drainage.

Main Results:

  • * Over 1,000 cervical heart transplants performed with a 95% success rate.
  • * The non-suture cuff technique demonstrated high patency rates and short operating times.
  • * Successful retrograde perfusion of the transplanted heart was achieved.

Conclusions:

  • * The described non-suture cuff technique is highly effective for murine cervical heart transplantation.
  • * This method provides a robust and reproducible model for in vivo transplantation research in mice.
  • * The technique's high success rate facilitates mechanistic studies using genetically diverse mouse strains.