Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

5.8K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Soluble Receptor for Advanced Glycation End Products (sRAGE) Is a Sensitive Biomarker in Human Pulmonary Arterial Hypertension.

International journal of molecular sciences·2021
Same author

Rapid Prototyping of Multilayer Microphysiological Systems.

ACS biomaterials science & engineering·2021
Same author

Cholinergic Activation of Primary Human Derived Intestinal Epithelium Does Not Ameliorate TNF-α Induced Injury.

Cellular and molecular bioengineering·2020
Same author

Effect of Monocyte Seeding Density on Dendritic Cell Generation in an Automated Perfusion-Based Culture System.

Biochemical engineering journal·2020
Same author

Automated generation of immature dendritic cells in a single-use system.

Journal of immunological methods·2018
Same author

Regeneration of glycocalyx by heparan sulfate and sphingosine 1-phosphate restores inter-endothelial communication.

PloS one·2017
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Apr 21, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

17.4K

Perspective on microfluidic cell separation: a solved problem?

Brian D Plouffe1, Shashi K Murthy

  • 1Department of Chemical Engineering and Barnett Institute of Chemical and Biological Analysis, Northeastern University , Boston, Massachusetts 02115, United States.

Analytical Chemistry
|October 29, 2014
PubMed
Summary
This summary is machine-generated.

Microfluidic cell separation technologies, including fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS), are maturing rapidly. These advancements offer new possibilities for clinical diagnostics and therapeutic monitoring, particularly for circulating tumor cells.

More Related Videos

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

8.3K
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

30.0K

Related Experiment Videos

Last Updated: Apr 21, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

17.4K
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

8.3K
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

30.0K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Microfluidic cell separation is a rapidly advancing research area.
  • Driven by clinical diagnostics and therapeutic monitoring needs, especially for circulating tumor cells.
  • Significant progress has been made in various separation techniques.

Purpose of the Study:

  • To review major scientific and technological advances in microfluidic cell separation.
  • To discuss the future outlook and challenges in this mature field.

Main Methods:

  • Miniaturized analogs of established techniques like fluorescence- and magnetic-activated cell sorting (FACS and MACS).
  • Specialized approaches utilizing cell affinity, dielectrophoretic mobility, and inertial properties.
  • Analysis of technological maturity and commercialization trends.

Main Results:

  • A broad range of microfluidic cell separation modalities have been developed.
  • Several technologies are nearing commercialization, indicating field maturity.
  • The field faces unique challenges despite scientific advancements.

Conclusions:

  • Microfluidic cell separation has matured significantly in a short period.
  • Future research will address remaining scientific questions and emerging challenges.
  • The field's maturity presents both opportunities and unique hurdles for development and application.