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Updated: Apr 21, 2026

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
Published on: April 15, 2021
The complete mitochondrial DNA genomes for two lineages of Aedes notoscriptus (Diptera: Culicidae)
C M Hardy1, L N Court1, M J Morgan1
1a CSIRO Land & Water Flagship , Clunies Ross Street , Canberra , ACT , Australia and.
Abstract:
The complete mitochondrial genomes for two deeply divergent lineages of the urban adapted mosquito Aedes notoscriptus Skuse (Diptera: Culicidae) in Australia were sequenced using a combination of next generation Illumina and traditional Sanger sequencing. The 15,846 and 15,851 bp circular genomes share 95.0% nucleotide identity. They both have the full complement of 37 metazoan genes and identical gene arrangements to previously published Culicidae species with the one non-coding A + T rich control region present between rns and tRNA-Ile. All protein initiation codons are ATN apart from COX1 (TCG). Eight protein coding genes encode full TAA stop codons, one uses an incomplete TA and four use T. Typical cloverleaf structures containing DHU and TΨC stem and loops can be inferred for all 22 tRNAs.

