Related Experiment Video
Updated: Apr 21, 2026

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
Published on: June 30, 2021
A ratiometric fluorescent probe with unexpected high selectivity for ATP and its application in cell imaging
Jia-Liang Tang1, Chun-Yan Li, Yong-Fei Li
1Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, P. R. China. lichunyan79@sina.com.
Abstract:
Naphthalimide-rhodamine compound (NR) is developed as a ratiometric fluorescent probe for ATP detection based on the FRET mechanism. It shows an unexpected high selectivity for ATP over other anions, especially organic phosphate anions, due to simultaneous interactions of two recognition sites, which benefits fluorescence imaging in living cells.

