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Updated: Apr 21, 2026

Measuring Local Anaphylaxis in Mice

Measuring Local Anaphylaxis in Mice

Published on: October 14, 2014

Measuring local anaphylaxis in mice.

Holly Evans¹, Kristin E Killoran¹, Edward Mitre²

¹Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences.

Journal of Visualized Experiments : Jove

|October 29, 2014

Summary

This summary is machine-generated.

This study details a mouse model to measure allergic responses by quantifying vascular permeability. The method uses Evans Blue dye to visualize and quantify localized allergic reactions in ear tissue.

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Last Updated: Apr 21, 2026

Measuring Local Anaphylaxis in Mice

Measuring Local Anaphylaxis in Mice

Published on: October 14, 2014

Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation

Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation

Published on: September 14, 2018

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice

Published on: April 13, 2010

Area of Science:

Immunology
Allergy Research
Animal Models

Background:

Allergic responses involve mast cell and basophil activation, releasing mediators that cause symptoms from erythema to anaphylaxis.
Understanding allergic mechanisms requires robust laboratory models.

Purpose of the Study:

To describe a detailed method for quantifying localized allergic responses by measuring changes in vascular permeability.
To adapt and refine the local anaphylaxis assay for reliable assessment of allergic reactions.

Main Methods:

Sensitization of mice to ovalbumin (OVA).
Challenging one ear with OVA and the other with vehicle.
Intravenous injection of Evans Blue dye, followed by euthanasia, dye extraction, and spectrophotometric quantification.

Main Results:

The assay provides a visual and quantifiable measure of localized allergic responses.
Demonstrates reliable detection of vascular permeability changes indicative of allergic reactions.

Conclusions:

The described local anaphylaxis assay is an effective method for quantifying allergic responses in a laboratory setting.
This method aids in understanding the mechanisms of allergic reactions.