Related Experiment Video

Updated: Apr 21, 2026

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

4.4K

Novel antiseptic compound OPB-2045G shows potent bactericidal activity against methicillin-resistant Staphylococcus

Yasuhide Inoue1, Akifumi Hagi1, Takuya Nii1

  • 1Naruto Research Institute, Research and Development Centre, Otsuka Pharmaceutical Factory Inc., Tokushima, Japan.

Journal of Medical Microbiology
|October 30, 2014
PubMed

Insights

A new compound, OPB-2045G, demonstrates potent bactericidal activity against resistant bacteria like MRSA and VRE. This novel agent shows promise in combating health care-associated infections more effectively than current treatments.

Area of Science:

  • Antimicrobial resistance
  • Infectious diseases
  • Pharmacology

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) pose significant threats in healthcare settings.
  • Existing treatments are becoming less effective against these multidrug-resistant pathogens.
  • Novel antimicrobial compounds are urgently needed to combat these infections.

Purpose of the Study:

  • To evaluate the bactericidal potency of the novel monobiguanide compound OPB-2045G.
  • To compare the efficacy of OPB-2045G against MRSA and VRE with established antiseptics: chlorhexidine digluconate (CHG) and povidone iodine (PVP-I).

Main Methods:

  • In vitro analysis using minimum bactericidal concentration (MBC) to determine bactericidal activity.
  • In vivo assessment of bactericidal efficacy in a mouse model of skin contamination with MRSA and VRE.
  • Comparison of OPB-2045G at a clinically relevant dose (1.5%) against CHG (0.5%) and PVP-I (10%).

Main Results:

  • OPB-2045G exhibited lower MBC values than CHG and PVP-I against MRSA and VRE strains.
  • In vivo, OPB-2045G showed superior bactericidal activity against MRSA compared to CHG and was comparable to PVP-I.
  • OPB-2045G demonstrated higher in vivo bactericidal activity against VRE than both CHG and PVP-I.

Conclusions:

  • OPB-2045G displays potent in vitro and in vivo bactericidal activity against MRSA and VRE.
  • The novel compound OPB-2045G offers a promising alternative for preventing and treating infections caused by these challenging pathogens.
  • OPB-2045G may enhance protection against health care-associated infections.

More Related Videos

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

4.1K
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

24.2K

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
74
Hand hygiene01:23

Hand hygiene

Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
5.1K
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
2.3K
Chemical Agents for Microbial Control01:27

Chemical Agents for Microbial Control

Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
1.6K
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
146
Surface Membrane Barriers01:18

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
2.9K