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Updated: Apr 21, 2026

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
Published on: June 25, 2013
RNA-directed recombination of RNA in vitro
Niles Lehman1, Nilesh Vaidya, Jessica A M Yeates
1Department of Chemistry, Portland State University, 751, Portland, OR, 97207, USA, niles@pdx.edu.
Abstract:
Construction of long RNAs can be achieved in vitro by using ribozymes to recombine shorter RNAs. This can be a useful technique to prepare RNAs when the final product is either very long or contains chemical modifications that are difficult to incorporate using standard in vitro transcription techniques. Here, we describe the use of the Azoarcus group I intron ribozyme to recombine shorter RNAs into longer ones. This ribozyme is a generalized RNA recombinase ribozyme that operates rapidly and with high efficiency.
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