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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

Published on: July 22, 2022

New approach to capture and characterize synaptic proteome.

Xin-An Liu¹, Beena Kadakkuzha¹, Bruce Pascal²

¹Department of Neuroscience.

Proceedings of the National Academy of Sciences of the United States of America

|October 30, 2014

Summary

Researchers identified proteins transported to synapses by kinesin (Kif) complexes. This study reveals kinesins

Keywords:

hippocampus kinesin protein transport proteome synapse

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Published on: May 12, 2017

Area of Science:

Neuroscience
Molecular Biology
Proteomics

Background:

The precise protein composition of synapses, crucial for neuronal communication, remains largely unknown.
Understanding synaptic protein transport is key to deciphering neuronal function and dysfunction.

Purpose of the Study:

To develop and apply a proteomic strategy for identifying proteins transported by molecular motor kinesins to synapses.
To compare the synaptic protein cargos transported by kinesin heavy chain 5C (Kif5C) and kinesin family member 3A (Kif3A) in the mouse brain.

Main Methods:

Isolation and systematic proteomic characterization of kinesin complexes (Kif5C and Kif3A).
Quantitative proteomics to identify and compare protein cargos associated with each kinesin in mouse hippocampus and prefrontal cortex.

Main Results:

Approximately 40-50% of identified kinesin-associated proteins are known synaptic proteins.
The specific kinesin complex and its expression location dictate the identity of transported proteins.
Significant overlap observed between kinesin cargos and proteins localized at synapses.

Conclusions:

Kinesins play a critical, previously underappreciated role in regulating synaptic proteome composition.
This proteomic approach provides novel insights into the molecular mechanisms of synaptic protein trafficking.