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KGFR promotes Na+ channel expression in a rat acute lung injury model
Binjian Liu1, Xin Lü2, Chaoling Qi1
1Laboratory department, 161st Central Hospital of P.L.A., Wuhan 430010, Hubei Province, China.
Background:
Binding of keratinocyte growth factor (KGF) to the KGF receptor (KGFR) plays an important role in the recovery of alveolar epithelial cells from acute lung injury (ALI).
Objectives:
To evaluate the effect of gene therapy via adenovirus gene transfer of KGFR on the treatment of ALI.
Methods:
Sprague-Dawley rats were divided into four groups: normal controls, injury controls, normal adenovirus transduced group and injury adenovirus transduced group. The ALI model was induced by lipopolysaccharide (LPS) injection. Recombinant adenovirus (AdEasy-KGFR) was injected via the tail vein. Expression of the sodium (Na(+)) channel in rat alveolar type II (ATII) epithelial cells was determined by PCR, immunohistochemistry and immunoelectron microscopy of rat lung tissues.
Results:
Gene expression of the Na(+) channel and KGFR in ATII cells was higher in the normal adenovirus transduced group than the three other groups; expression of these two genes in the injury adenovirus transduced group was higher than the injury control group. Na(+) channel protein expression was lower in the injury adenovirus transduced group but higher than the injury control group.
Conclusions:
KGFR over-expression induced Na channel expression could potentially be beneficial for ALI therapy.
